Journal: Neurochemical Research

Article Title: Plasma-Derived Exosomal hsa-miR-3677-3p Induces Ferroptosis in Neurons by Targeting ABCB8 in Perioperative Neurocognitive Disorders After Prostate Surgery

doi: 10.1007/s11064-026-04665-2

Figure Lengend Snippet: Upregulation of exosomal hsa-miR-3677-3p in dNCR patients and its targeting of ABCB8. A qRT-PCR analysis of hsa-miR-3677-3p levels in plasma-derived exosomes from dNCR patients compared to the control group ( n = 15/group). hsa-miR-3677-3p showed a significant upregulation of 2.236 ± 0.6160-fold change ( p = 0.0011). B Receiver operating characteristic (ROC) curve analysis demonstrating the diagnostic potential of hsa-miR-3677-3p in plasma-derived exosomes from dNCR patients compared to the control group. The area under the curve (AUC) is 0.83 (95% CI: 0.6875–0.9747, p = 0.002). C Schematic representation of the dual-luciferase reporter construct, featuring the SV40 promoter driving firefly luciferase expression, followed by the 3′ UTR of ABCB8 containing predicted binding sites for hsa-miR-3677-3p. D Relative luciferase activity in 293 T cells co-transfected with either the wild-type (WT) or mutant (MUT) 3′-UTR of ABCB8, along with hsa-miR-3677-3p mimics, inhibitors, or controls ( n = 3/group). E qRT-PCR analysis evaluating ABCB8 mRNA expression following the transfection of SH-SY5Y cells with hsa-miR-3677-3p mimics, inhibitors, or negative controls (NC and Inhibitor-NC) ( n = 3/group). Transfection with mimics significantly reduced ABCB8 mRNA levels by 3.05 ± 0.1029-fold change ( p = 0.0046), while inhibitors increased expression by 1.82 ± 0.1595-fold change ( p = 0.0055), compared to their respective negative controls. F Representative images of western blot analysis showing ABCB8 protein levels in SH-SY5Y cells after the transfection of SH-SY5Y cells with hsa-miR-3677-3p mimics, inhibitors, or negative controls (NC and Inhibitor-NC). G Quantification of ABCB8 protein levels from western blot analysis, normalized to β-ACTIN as an internal control ( n = 6/group). Mimic transfection reduced protein expression by 2.28 ± 0.0658-fold ( p < 0.001), whereas inhibitor transfection increased expression by 1.72 ± 0.4141-fold ( p < 0.001), compared to respective controls. Statistical tests: A Student’s t-test; B DeLong’s test for ROC curve comparison; D , E , and G one-way ANOVA with Tukey’s multiple comparisons test. Error bars denote SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: At 24- or 48-hours post-transfection, cells were lysed, and luminescence was quantified using the Dual-Luciferase Reporter Assay System (DL101, Vazyme, China).

Techniques: Quantitative RT-PCR, Clinical Proteomics, Derivative Assay, Control, Diagnostic Assay, Luciferase, Construct, Expressing, Binding Assay, Activity Assay, Transfection, Mutagenesis, Western Blot, Comparison